Liquid–liquid phase coexistence in lipid membranes observed by natural abundance 1H–13C solid-state NMR
| Autor: | Tiago Mendes Ferreira, Lucas Löser, Kay Saalwächter |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Isotope Liquid ordered phase Abundance (chemistry) Chemistry Analytical chemistry General Physics and Astronomy Isotopic labeling 03 medical and health sciences 030104 developmental biology Membrane Pulmonary surfactant Solid-state nuclear magnetic resonance Phase (matter) lipids (amino acids peptides and proteins) Physical and Theoretical Chemistry |
| Zdroj: | Physical Chemistry Chemical Physics. 20:9751-9754 |
| ISSN: | 1463-9084 1463-9076 |
| Popis: | We demonstrate that 1H–13C solid-state MAS NMR is suitable to detect liquid disordered/liquid ordered phase coexistence in a DOPC/DPPC/cholesterol mixture with natural abundance of isotopes as an alternative to 2H NMR. Such methodology is potentially applicable to study lipid phase coexistence phenomena in biological matter with high lipid content, e.g. lung surfactant or myelin, for which isotopic labeling is not possible. |
| Databáze: | OpenAIRE |
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