Rapid isolation and HPTLC validated method for the quantification of echioidinin-5-O-β-d-glucopyranoside inAndrographis echioides

Autor: Balu V. Ghule, Pramod G. Yeole
Rok vydání: 2012
Předmět:
Zdroj: Journal of Planar Chromatography – Modern TLC. 25:575-580
ISSN: 1789-0993
0933-4173
DOI: 10.1556/jpc.25.2012.6.15
Popis: Separation of echioidinin-5-O-β-d-glucopyranoside (ECG) from Andrographis species of plants by column chromatographic techniques had various shortcomings. Therefore, we report a rapid and efficient method to isolate ECG from ethyl acetate fraction of petroleum ether defatted methanol extract of Andrographis echioides (AE) aerial parts. The isolated and purified ECG was identified by melting point, UV, FTIR, 1H- and 13C-NMR and mass spectroscopy studies and used to develop a simple and reproducible HPTLC method for its determination in AE. The method was validated for linearity, precision, robustness, specificity, accuracy, and limits of detection and quantification. The method employed TLC aluminum plates precoated with silica gel 60 F254 as the stationary phase and chloroform-methanol (77.5:22.5, v/v) as a mobile phase. A CAMAG TLC Scanner 3 was used for spectrodensitometric scanning and analysis at 254 nm. The linear regression analysis data for the calibration plot shows good linearity relationship wit...
Databáze: OpenAIRE
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