Adoptive immunotherapy with antigen-specific T cells during extracorporeal membrane oxygenation (ECMO) for adenovirus-related respiratory failure in a child given haploidentical stem cell transplantation
| Autor: | Nicola Pirozzi, Alice Bertaina, Sergio Rutella, Ezio Giorda, Matteo Di Nardo, Letizia Pomponia Brescia, Stefano Ceccarelli, Corrado Cecchetti, Antonio Amodeo, Franco Locatelli, Giuseppina Li Pira |
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| Rok vydání: | 2013 |
| Předmět: |
business.industry
medicine.medical_treatment virus diseases Hematology Immunotherapy Hematopoietic stem cell transplantation medicine.disease Virology eye diseases Transplantation Immune system Oncology Antigen Pediatrics Perinatology and Child Health Immunology medicine Extracorporeal membrane oxygenation Congenital amegakaryocytic thrombocytopenia Stem cell business |
| Zdroj: | Pediatric Blood & Cancer. 61:376-379 |
| ISSN: | 1545-5009 |
| Popis: | We report on the successful infusion of human adenovirus (HAdV)-specific T cells in a child with congenital amegakaryocytic thrombocytopenia, given T-cell-depleted hematopoietic stem cell transplantation (HSCT) from the HLA-haploidentical mother during extracorporeal membrane oxygenation (ECMO) for severe HAdV-related respiratory failure. Donor-derived, interferon (IFN)-γ-secreting HAdV-specific T cells were enriched using the cytokine capture assay, after in vitro stimulation with overlapping peptides from the immunodominant HAdV5 hexon protein. Two weeks after T-cell transfer, viral load decreased and ECMO was discontinued. T-cell responses to HAdV antigens were documented after four weeks and were associated with viral clearance, immune reconstitution and clinical amelioration. Pediatr Blood Cancer 2014;61:376–379. © 2013 Wiley Periodicals, Inc. |
| Databáze: | OpenAIRE |
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