| Autor: |
Bill Chaudhry, Pascal Verdonck, Patrick Segers, P. Van Ransbeeck, Frédéric Maes |
| Rok vydání: |
2009 |
| Předmět: |
|
| Zdroj: |
IFMBE Proceedings ISBN: 9783540892076 |
| DOI: |
10.1007/978-3-540-89208-3_446 |
| Popis: |
Heart defects remain the most common birth defect in humans. The majority of the defects are believed to be influenced by both genetic patterning and mechanical stimuli such as the shear stress exerted by the blood flow on the developing heart. In order to better understand the relationship between heart development and wall shear stress, we aim to explore the structure and function of the embryonic heart, assess the shear stress patterns and investigate the relation between both throughout embryonic heart development. Using transmission light microscopy in combination with high speed image recording modalities we have succeeded in mapping intracardiac flow in vivo in the zebrafish embryo. By tracking individual red blood cells it has been possible to reconstruct an instantaneous velocity field. Peak velocity of individual blood cells is in the order of 0.5mm/s. As the intracardiac flow is caused by the heart wall movement, it is important to understand the pumping mechanism of the embryonic heart. Therefore we also tracked myocardial wall cells, demonstrating a peristaltic like motion. Microscopic observation of blood flow and wall motion in the embryonic heart is feasible, and will provide the necessary input for further modeling studies. We will use CFD (computational fluid dynamics) to provide more detailed information on flow velocity distribution and local wall shear stresses. In future experimental work, we will also disturb the hemodynamics and boundary conditions of the developing heart to generate disturbed intracardiac flow patterns. Modeling and follow up of these embryos should enhance our insight into the role of mechanical stimuli in congenital heart defects. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
