Application of continuous supercritical anti-solvents for rapid recrystallization and purification of zeaxanthin dipalmitates from de-glycosides of Lycium barbarum fruits
| Autor: | Shih-Lan Hsu, Bing-Chung Liau, Chieh-Ming J. Chang, Jia-Hui Cheng, Tzong-Ming Wu, Li-Pin Chang |
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| Rok vydání: | 2011 |
| Předmět: |
chemistry.chemical_classification
Recrystallization (geology) Chromatography biology Elution General Chemical Engineering Glycoside Fractionation Condensed Matter Physics biology.organism_classification Supercritical fluid Zeaxanthin chemistry.chemical_compound chemistry Acetone Lycium Physical and Theoretical Chemistry |
| Zdroj: | The Journal of Supercritical Fluids. 57:155-161 |
| ISSN: | 0896-8446 |
| DOI: | 10.1016/j.supflu.2011.02.008 |
| Popis: | This study employed column elution fractionation coupled with supercritical anti-solvent recrystallization to isolate the purest zeaxanthin dipalmitates (ZP), one of the eye-protecting compounds in the Lycium barbarum fruits. The amount of ZP increased to 983 mg/g in liquid anti-solvent precipitates with 29.1% recovery and increased to 929 mg/g in supercritical anti-solvent precipitates with 59.6% recovery, respectively from the ultrasonic acetone extract. Experimental data showed that supercritical anti-solvent recrystallization is feasible in producing platelet type precipitates within a few minutes. These ZP compounds exhibit anti-oxidative abilities by proliferating human adult retinal pigment epithelial cells (from 22% to 31%); though it was a meager increase when compared to those of algal 95% zeaxanthin (from 43% to 94%). |
| Databáze: | OpenAIRE |
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