| Autor: |
Djamel Saidi, F. Mezemaze, H. Negaoui, Omar Kheroua, A. Chekroun, H. Kaddouri, K. E. El-Mecherfi, N. Youcef |
| Rok vydání: |
2009 |
| Předmět: |
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| Zdroj: |
American Journal of Applied Sciences. 6:1448-1452 |
| ISSN: |
1546-9239 |
| DOI: |
10.3844/ajassp.2009.1448.1452 |
| Popis: |
Problem statement: Our aim was to enhance the data on antigenic properties of dromedary whey proteins. Approach: The identification of the whey proteins was carried by SDS-page and Reversed phase high performance liquid chromatography (RP-HPLC). The cross-reactivity of dromedary whey proteins with IgG anti bovine β-lactoglobulin and anti bovine α-lactalbumin, obtained by immunisation of Balb/c mice, was carried out by ELISA. Results: The SDS-page showed the presence of band corresponding to α-lactalbumin and albumin serum; this was confirmed by the chromatogram obtained by RP-HPLC, where we detected a pick corresponding to α-lactalbumin. There was a cross reaction of dromedary whey proteins with IgG anti bovin α-?lactalbumin but it was very weak with IgG anti boin β-lactoglobulin. Conclusion: We detected a cross reactivity between dromedary whey proteins and IgG anti bovin α-lactalbumin. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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