Mutagenesis, Screening and Industrialization of High Avermectin B1a Producing Strains from Streptomyces Avermitilis Irradiated by 12C 6+ Ion Beam

Autor: Jian Ping Liang, Xiao Qi Ma, Jing Liu, Yong Heng Bo, Shu Yang Wang, Wen Jiang Li, Ji Hong Chen
Rok vydání: 2011
Předmět:
Zdroj: Advanced Materials Research. 339:652-655
ISSN: 1662-8985
DOI: 10.4028/www.scientific.net/amr.339.652
Popis: Heavy ion irradiation induced mutation is a unique method of physical mutation, with various parameters, high LET and RBE characteristic. The spore suspension of streptomyces avermitilis were irradiated by carbon ions with doses of 0、30、40、50、60 and 70 Gy. The high avermectin producing strain was successfully obtained after the mutagenesis processing by heavy ion beam and the screening of orthomutation strains. Two strains(No.203 and No.148) which potency was 5200μg/ml was sieved and its potency increased 38.64% than the original strain. The obtained strains were used for 1 ton fermentation tank and the pilot 5-ton fermentation tank amplification tests.
Databáze: OpenAIRE