A rapid, sensitive and field-deployable isothermal assay for the detection of Verticillium alfalfae
| Autor: | Jerry E. Weiland, Jeremiah K. S. Dung, Andres Reyes Gaige |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine fungi Verticillium alfalfae food and beverages Wilting Taproot Plant Science Biology Verticillium biology.organism_classification 01 natural sciences Crop 03 medical and health sciences medicine.drug_formulation_ingredient Horticulture 030104 developmental biology Shoot medicine Cultivar Verticillium wilt Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Canadian Journal of Plant Pathology. 40:408-416 |
| ISSN: | 1715-2992 0706-0661 |
| DOI: | 10.1080/07060661.2018.1474262 |
| Popis: | The genus Verticillium contains 10 plant pathogenic species that are responsible for billions of dollars of crop losses annually on more than 400 plant species worldwide. On alfalfa, Verticillium alfalfae causes Verticillium wilt, a disease with the potential to reduce yields of susceptible cultivars by the second harvest year and to limit the productive stand life to less than 3 years. Susceptible alfalfa cultivars exhibit disease symptoms that include chlorotic V-shaped lesions at the leaf tip that cause entire leaflets to become bleached and twisted, leading to defoliation. Stems often remain green after all leaflets have desiccated, and discolouration, stunting and wilting of shoots is visible. Internally, the taproot can show yellow to brown vascular discolouration. International quarantines sometimes prohibit the entry of alfalfa samples contaminated with the Verticillium pathogen. In this investigation, we developed a recombinase polymerase amplification (RPA) assay coupled with a lateral f... |
| Databáze: | OpenAIRE |
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