Improved fluorescence polarization assay for use in evaluating fetal lung maturity. III. Retrospective clinical evaluation and comparison with the lecithin/sphingomyelin ratio
| Autor: | Edward R. Ashwood, C A Foerder, T J Benedetti, R W Franklin, J F Tait |
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| Rok vydání: | 1986 |
| Předmět: |
Pathology
medicine.medical_specialty Amniotic fluid Respiratory distress business.industry Biochemistry (medical) Clinical Biochemistry Reference range Transient tachypnea of the newborn Lecithin–sphingomyelin ratio medicine.disease Andrology Meconium Medicine business Prospective cohort study Fluorescence anisotropy |
| Zdroj: | Clinical Chemistry. 32:260-264 |
| ISSN: | 1530-8561 0009-9147 |
| DOI: | 10.1093/clinchem/32.2.260 |
| Popis: | We clinically evaluated, retrospectively, our improved fluorescence polarization assay for fetal lung maturity. The procedure requires 0.5 mL of amniotic fluid and a standard clinical laboratory fluorescence polarimeter (TDx Analyzer, Abbott Laboratories). We measured the L/S ratios for 93 freshly collected amniotic fluids, uncontaminated with blood or meconium, collected within three days of delivery. The fluids were stored frozen for eight to 32 months, then thawed and assayed for net fluorescence polarization. Fourteen of the infants developed respiratory distress syndrome; five, transient tachypnea of the newborn; and 74, no respiratory distress. The polarization assay and lecithin/sphingomyelin ratio had equivalent receiver operating characteristic curves, indicating no difference in their clinical performance. Although a prospective study with fresh amniotic fluid specimens will be necessary to establish a definitive reference range, the present study shows that this assay can be used to rapidly predict fetal lung maturity. |
| Databáze: | OpenAIRE |
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