SULFATED DERIVATIVES OF ARABINOGALACTAN AND THEIR ANTICOAGULANT ACTIVITY
| Autor: | M. A. Mikhailenko, Tatyana P. Shakhtshneider, Natal'ya Yur'yevna Vasil'yeva, Boris N. Kuznetsov, Nikolay V. Chesnokov, Yuriy N. Malyar, Svetlana A. Kuznetsova, Natal'ya Nikolayevna Drozd |
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| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
Molecular mass Sodium Organic Chemistry Dispersity chemistry.chemical_element 02 engineering and technology Plant Science 021001 nanoscience & nanotechnology Biomaterials 03 medical and health sciences chemistry.chemical_compound Sulfation chemistry Arabinogalactan parasitic diseases Sulfamic acid Molar mass distribution Ammonium 0210 nano-technology 030304 developmental biology Nuclear chemistry |
| Zdroj: | chemistry of plant raw material. :47-56 |
| ISSN: | 1029-5143 1029-5151 |
| DOI: | 10.14258/jcprm.2019044845 |
| Popis: | The IR spectra and molecular mass distribution of arabinogalactan sulfates in the form of sodium and ammonium salts, obtained using various sulfating reagents, were compared. According to the obtained data, the sulfated derivatives of arabinogalactan differ from each other by the nature of the hydrogen bonds and the molecular weight distribution. Using coagulological tests at the activation of coagulation of platelet-poor human plasma, in vitro study of the anticoagulant properties of arabinogalactan sulfated derivatives in various salt forms differing in methods of preparation, degree of sulfation and molecular weight was conducted. It was established that the sample in the form of arabinogalactan sodium salt (SAG 1) with a sulfur content of 13.2 wt.% аnd a polydispersity degree of 1.52 showed 2 times more anticoagulant activity than the sample in the form of ammonium salt of arabinogalactan (SAG 2) with the sulfur content of 6.6 wt.% and the degree of polydispersity of 1.30. Antithrombin (aIIa) activity of samples obtained by sulfation with pyridine and sulfuric anhydride complex (SAG 1) and sulfamic acid complex (SAG 2) was, respectively, 23.42±1.86 and 10.20±1.50 U/mg; the anti-factor Xa activity of SAG 1 and SAG 2 was 2.13±0.42 and 0.37±0.08 U/mg; and the ratio aIIa/aXa for SAG1 and SAG 2 was 11 and 28, respectively. The less activity of antifactor Xa(aXA) of SAG as compared to unfractionated heparin (UFG) and higher rations of activities aIIa/aXa may contribute the less provocation of bleeding by SAG samples in the comparition with UFG. |
| Databáze: | OpenAIRE |
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