Application De La MÉthode De Kunkel Au Dosage Des Gamma-Globulines Dans Les Serums Hyperglobuliniques De MyÉlomateuxpar
| Autor: | Fr. Gregoire, Verbanck M, De Heinzelin De Braucourt C, Lambert Pp |
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| Rok vydání: | 1952 |
| Předmět: | |
| Zdroj: | Acta Clinica Belgica. 7:144-147 |
| ISSN: | 2295-3337 1784-3286 |
| DOI: | 10.1080/17843286.1952.11717207 |
| Popis: | SummaryKunkel’s technic for precipitation of globulins was applied to hyperglobulinic serum of patients with multiple myeloma-with following results :Turbidity was in those cases too high to allow comparison with the McLaghan's SO4Ba scale.Dilution of the serum or precipitation with larger amounts of Kunkel’s reagent showed obvious errors in the dosage of globulins (Fig. 1).Further investigations made possible to understand the mechanism of these erroneous results.First, a linear correlation was noticed between photometric readings and concentration of pure globulins solutions (Fig. 2).Thereafter, several mixtures of pure albumin and globulins were prepared, definited by' their A/G ratio; they were diluted and precipitated by Kunkel’s reagent. Authors found a curvilinear relation of readings with globulin concentration (Fig. 3).Low levels of serumalbumins increased the zinc turbidity values. High levels inhibited the floculation of globulins. The lower the A/G ratio of the mixture, the highest are the amo... |
| Databáze: | OpenAIRE |
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