Modification of a P-Phenylenediamine Oxidase Method to Permit Non-Automated Ceruloplasmin Determinations in Batches of Rat Serum or Plasma Microsamples

Autor:	James M. Boiano, Harold G. Petering, Edward E. Menden, Lalitha Murthy
Rok vydání:	1977
Předmět:	Oxidase test Chromatography biology Plasma samples Chemistry Batch method Biochemistry (medical) Clinical Biochemistry p-Phenylenediamine Plasma P-phenylenediamine oxidase Biochemistry Analytical Chemistry chemistry.chemical_compound Electrochemistry biology.protein Ceruloplasmin Spectroscopy
Zdroj:	Analytical Letters. 10:197-204
ISSN:	1532-236X 0003-2719
DOI:	10.1080/00032717708059204
Popis:	An essentially one-sample-at-a time p-phenylenediamine oxidase method was converted to a batch method by employing 0°C temperature during the serum or plasma sample preparation for incubation at 37°C. The samples were also amenable to a simple scale-down of all reaction mixture components, so that good results could be obtained with samples ranging down to 0.010 ml from the usual 0.100 ml. These two modifications should be applicable to most p-phenylenediamine oxidase ceruloplasmin methods.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a8de5b775b73250877ddf40230667252 https://doi.org/10.1080/00032717708059204 Zobrazit plný text záznamu