Phosphorylation of histone H2AX in human lymphocytes as a possible marker of effective cellular response to ionizing radiation

Autor:	Andrey Kropotov, Nikolai V. Tomilin, Denis Firsanov
Rok vydání:	2011
Předmět:	Programmed cell death cells Histone H2AX Chromosome Cell Biology Biology Molecular biology Chromatin Blot enzymes and coenzymes (carbohydrates) chemistry.chemical_compound chemistry Histone H2A Phosphorylation biological phenomena cell phenomena and immunity DNA
Zdroj:	Cell and Tissue Biology. 5:531-535
ISSN:	1990-5203 1990-519X
DOI:	10.1134/s1990519x1106006x
Popis:	DNA double-strand breaks (DSBs) which occurs in cells after ionizing radiation (IR) or chemical agents are the most dangerous lesions in eukariotic cells, which leads to cell death or chromosome abberations and cancer. One of the earliest response of cells to DSBs formation is phosphorylation by 139 serine of core variant histone H2AX in megabase chromatin domains around DSB (gamma-H2AX), which amplify signal and makes it possible to identify even one DSB in genome. Effective formation of gamma-H2AX is very important for maintenance of genome stability. Here, using immunofluorescent and Western blotting techniques, we studied dynamics of gamma-H2AX formation in human lymphocytes of various individuals irradiated ex vivo. We have found that dynamics of gamma-H2AX formation in lymphocytes differ between individuals but have similar kinetics and statistically is independent on people age.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a8da6ad1941f78d4cf2634bd9878746e https://doi.org/10.1134/s1990519x1106006x Zobrazit plný text záznamu Full text from SpringerLink