Autor: |
Thurner, Marco, Deutsch, Martin, Janke, Katrin, Messner, Franka, Kreutzer, Christina, Beyl, Stanislav, Couillard-Després, Sébastien, Hering, Steffen, Troppmair, Jakob, Marksteiner, Rainer |
Rok vydání: |
2020 |
DOI: |
10.6084/m9.figshare.12476840.v1 |
Popis: |
Additional file 4: S4 Fig. Characterization of murine MPC and MPC-SMC. (A) AChE and (B) CK activity was measured by enzyme kinetics and is represented by OD412nm and OD340nm at specific time points (AChE: 60 min, CK: 10 min). Enzyme activities were compared between skeletal muscle-derived mMPC and non-myogenic cells after 6 days in skeletal muscle differentiation medium. (C) Desmin expression of mMPC visualized by immunofluorescence staining. (D) Formation of multinucleated myotubes by mMPC during differentiation in skeletal muscle differentiation medium for six days in vitro was observed. TdTomato and nuclei were stained on histological cross sections of tibialis anterior muscles of (E) control untreated mice and (F) SHO mice 70 days after intramuscular injection with TdTomato mMPC and fluorescent beads. Scale bar = 100 μm. |
Databáze: |
OpenAIRE |
Externí odkaz: |
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