New green fluorescent protein genes for plant transformation: Intron-containing, ER-localized, and soluble-modified

Autor:	William F. Thompson, S. Luke Mankin
Rok vydání:	2001
Předmět:	Reporter gene fungi Intron Plant Science Protoplast Biology Proteomics Plant cell Molecular biology Green fluorescent protein Cell biology Transformation (genetics) Molecular Biology Gene
Zdroj:	Plant Molecular Biology Reporter. 19:13-26
ISSN:	1572-9818 0735-9640
DOI:	10.1007/bf02824074
Popis:	We describe several new modifications of theAequorea victoria green fluorescent protein (GFP) gene. TheerGFP5INTreporter gene combines the PIV2 intron fromgusINTandLUCINTwith the ER-localizedmGFP4-ER gene. TheerGFP6INT, erGFP7INT, anderBFP8INTgenes also include the fluorophore and solubility modifications of smGFP, smRS-GFP, and smBFP, respectively. A parallel set of reporter genes (erGFP5, erGFP6, erGFP7, anderBFP8) is otherwise identical to the respectiveerGFPINTgenes but lacks the PIV2 intron. The intron-containing genes are expressed in plant cells but not in bacteria, allowing detection of plant cell expression in the presence ofAgrobacterium during the early stages of transformation. Transient expression of theerGFPINTanderGFP genes is comparable in tobacco and maize suspension culture protoplasts, indicating that the PIV2 intron is spliced effectively in both monocotyledonous and dicotyledonous plant species.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a8c65d6e20b1cf6a52f8af5f9ac061bb https://doi.org/10.1007/bf02824074 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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