Validation of a confirmatory analytical method for the determination of aflatoxins B1, B2, G1and G2in foods and feed materials by HPLC with on-line photochemical derivatization and fluorescence detection
| Autor: | Carmen Palermo, Diego Centonze, Sonia Lo Magro, Domenico Palermo, Marco Iammarino, Marilena Muscarella, Donatella Nardiello |
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| Rok vydání: | 2009 |
| Předmět: |
Aflatoxin
Chromatography Resolution (mass spectrometry) Chemistry Elution Health Toxicology and Mutagenesis Extraction (chemistry) Public Health Environmental and Occupational Health General Chemistry General Medicine Toxicology Photochemistry High-performance liquid chromatography chemistry.chemical_compound Sample preparation Derivatization Acetonitrile Food Science |
| Zdroj: | Food Additives & Contaminants: Part A. 26:1402-1410 |
| ISSN: | 1944-0057 1944-0049 |
| Popis: | A sensitive and selective analytical method for the simultaneous separation and quantitative determination of aflatoxins B1, B2, G1 and G2 in foodstuffs and materials for feed has been validated. The method is based on high performance liquid chromatography with on-line post-column photochemical derivatization and fluorescence detection. The chromatographic separation of aflatoxins was accomplished using a C18 column eluted with an isocratic mobile phase consisting of water, methanol and acetonitrile. The sample preparation required a simple extraction of aflatoxins with MeOH/H2O (80:20, v/v) and a purification step by immunoaffinity column cleanup. The total analysis time, including sample preparation and chromatographic separation, did not exceed 40 min with a run time of 10 min. The on-line photochemical derivatization ensures better results in terms of simplicity, sensitivity and reproducibility with respect to chemical derivatization techniques, and provides an increase of the peak resolution and an ... |
| Databáze: | OpenAIRE |
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