The Protective Effect of Silymarin on LipopolysaccharideInduced Liver Toxicity in Male Wistar Rat

Autor:	Samaneh Borooni, Elaheh Tajbakhsh, Fahimeh Nourbakhsh
Rok vydání:	2019
Předmět:	chemistry.chemical_compound Lipopolysaccharide chemistry Liver toxicity business.industry Medicine lipids (amino acids peptides and proteins) Pharmacology business
Zdroj:	International Journal of Medical Laboratory.
ISSN:	2423-3714 2423-3706
DOI:	10.18502/ijml.v6i4.2010
Popis:	Background and Aims: Lipopolysaccharide (LPS) triggers production of reactive oxygen species and inflammatory cytokines. Nowadays Silybum marianum has been shown to treat liver and gall bladder disorders, especially to protect the liver against poisoning from various toxins. Therefore, we decided to evaluate the protective effect of silymarin on LPS-induced liver toxicity in male Wistar rat. Materials and Methods: Totally, 40 male Wistar rats were divided into 4 groups (n=10 in each): The animals were treated with silymarin for two weeks before the biochemical tests. Apoptosis was assessed by evaluating the amount of proteins in liver tissues by western blotting. Results: LPS induced hepatotoxicity as evidenced by histopathological damages and biochemical abnormalities. Data showed that malondialdehyde level significantly increases in the liver of LPS-treated rats. Destructive effects of LPS on histopathological and biochemical parameters were improved. LPS also increased expression of Bax/Bcl2 ratio and activation of caspase 3, caspase 8 and caspase 9. Western blot analysis showed silymarin treatment inhibiting apoptosis stimulated by LPS in the liver (p
Databáze:	OpenAIRE
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