ß- Glucosidases from leaves and roots of the common beet,Beta vulgaris

Autor:	T. A. Petrova, N. S. Zakharova
Rok vydání:	2000
Předmět:	chemistry.chemical_classification Chromatography Size-exclusion chromatography Biology Applied Microbiology and Biotechnology Biochemistry Hydrolysis Membrane Enzyme chemistry Sephadex biology.protein Betacyanins Glucosidases Ammonium sulfate precipitation
Zdroj:	Applied Biochemistry and Microbiology. 36:394-397
ISSN:	1608-3024 0003-6838
Popis:	s-Glucosidases detected in the leaves and roots of the common beet,Beta vulgaris, have been demonstrated to catalyze hydrolysis of native betacyanins. A method is described for the isolation and purification of sglucosidase from the roots, which involves ammonium sulfate precipitation, DEAE-cellulose chromatography, and Sephadex gel filtration. Maximum activity of the enzyme is detected at 50°C and pH 8.0; it retains stability within the pH range from 5.1 to 9.2. In leaves, s-glucosidase is associated with chloroplast membranes; solubilization of the membranes results in enzyme inactivation.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a8aa9952c9960c460f6320cf62aee143 https://doi.org/10.1007/bf02738049 Zobrazit plný text záznamu Full text from SpringerLink