Characterization of a Corrinoid Compound from Pacific Bluefin Tuna (Thunnus orientalis) Liver
| Autor: | Yukinori Yabuta, Emi Imai, Shinya Masuda, Shigeo Takenaka, Yukio Ishihara, Fumio Watanabe, Tomoyuki Fujita |
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| Rok vydání: | 2011 |
| Předmět: |
Marketing
Vitamin Chromatography General Chemical Engineering Pacific bluefin tuna Microbiological assay Anatomy Biology biology.organism_classification Industrial and Manufacturing Engineering chemistry.chemical_compound Corrinoid chemistry Vitamin B12 Spectral data Tuna human activities Thunnus Food Science Biotechnology |
| Zdroj: | Food Science and Technology Research. 17:589-594 |
| ISSN: | 1881-3984 1344-6606 |
| DOI: | 10.3136/fstr.17.589 |
| Popis: | A substantial amount of vitamin B12 (357 ± 42.7 μg/100 g) was detected in pacific bluefin tuna (Thunnus orientalis) liver using a microbiological assay method. To evaluate whether tuna liver contains true vitamin B12 or an inactive corrinoid compound, a corrinoid compound was purified and characterized. The purified compound was identical to that of authentic vitamin B12, judging by TLC and LC/ESI-MS/MS chromatograms, and 1H NMR spectral data. These results indicate that pacific bluefin tuna liver contains substantial amounts of true vitamin B12 and would be an excellent source of the vitamin. |
| Databáze: | OpenAIRE |
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