Osmotic Swelling Activates two Pathways for K+ Efflux in a Rat Hepatoma Cell Line

Autor:	Pauline R. Junankar, Kiaran Kirk, Ari Karjalainen
Rok vydání:	2004
Předmět:	Taurine chemistry.chemical_compound Biochemistry Physiology Chemistry Osmolyte Cell culture Extracellular Biophysics Channel blocker Efflux Apamin Calcium-activated potassium channel
Zdroj:	Cellular Physiology and Biochemistry. 14:143-154
ISSN:	1421-9778 1015-8987
Popis:	The pathways for the efflux of K(+) from osmotically-swollen HTC rat hepatoma cells were investigated using (86)Rb(+) as a tracer for K(+). Exposure of HTC cells to a hypotonic solution ( 20 min. The second (86)Rb(+) efflux component was not inhibited by K(+) channel blockers but was inhibited by the anion channel blockers, tamoxifen, 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB) and niflumate. The time-courses for its activation and inactivation, as well as its dependence on the extracellular osmolality, were very similar to those observed for the hypotonically-activated efflux of the organic osmolyte, taurine. The data are consistent with the second component of (86)Rb(+) efflux and the efflux of taurine from osmotically-swollen cells occurring via a common pathway having a marked selectivity for taurine over (86)Rb(+).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a8842e590ca24e5c88955e41e2ac0b98 https://doi.org/10.1159/000078106 Zobrazit plný text záznamu