Endoglucanase Activity of Compost-Dwelling Fungus Paecilomyces inflatus is Stimulated by Humic Acids and Other Low Molecular Mass Aromatics
| Autor: | Beata Kluczek-Turpeinen, Pekka Maijala, Marja Tuomela, Annele Hatakka, Martin Hofrichter |
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| Rok vydání: | 2005 |
| Předmět: |
food.ingredient
Pectin Physiology Cellulase Cellobiose engineering.material complex mixtures Applied Microbiology and Biotechnology chemistry.chemical_compound food Botany Humic acid Food science Cellulose chemistry.chemical_classification biology Compost food and beverages General Medicine biology.organism_classification chemistry engineering biology.protein Fermentation Paecilomyces Biotechnology |
| Zdroj: | World Journal of Microbiology and Biotechnology. 21:1603-1609 |
| ISSN: | 1573-0972 0959-3993 |
| DOI: | 10.1007/s11274-005-8291-1 |
| Popis: | Paecilomyces inflatus isolated from municipal waste compost was found to have cellulolytic activity in several solid and liquid media. This study was done to reveal the multifarious effects of municipal waste compost on endoglucanase activity of P. inflatus. The highest enzyme activities under the conditions of solid-state fermentation were measured in authentic compost samples compared with wood, straw and bran substrates. In surface liquid cultures glucose, cellobiose, xylan, Avicel cellulose, carboxymethylcellulose (CM-cellulose), starch and citrus pectin were used as carbon sources. All carbon sources supported the growth of P. inflatus. However, only CM-cellulose, cellobiose and pectin noticeably stimulated endoglucanase (EG) activity. Further stimulation of EG activity was obtained in cultures containing 1% CM-cellulose as a carbon source by supplementation with low-molecular mass aromatic compounds vanillin, veratric acid and benzoic acid, and with soil humic acid (SHA). SHA and veratric acid were found to be the most efficient elicitors of the cellulolytic activity. P. inflatus was able to utilize nitrate and ammonium as pure nitrogen sources in media containing cellulose. |
| Databáze: | OpenAIRE |
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