Nomenclature standards and DNA barcoding of apple varieties originated by VIR Crimean Experimental Breeding Station

Autor: L. V. Bagmet, I. S. Chepinoga, A. A. Trifonova, K. V. Boris, A. V. Shlyavas
Rok vydání: 2021
Zdroj: Horticulture and viticulture. :5-16
ISSN: 2618-9003
0235-2591
DOI: 10.31676/0235-2591-2021-6-5-16
Popis: Current pace of breeding technologies relies on a tighter control of quality and identity of breeding achievements. Thereby, standardised variety nomenclature becomes of particular relevance to store original genetic information and warrant authenticity of the breeding product. A nomenclature standard regulates the variety’s name and habitus. A herbarium specimen is most appropriate for standardisation, since it defines a set of strongly inherited morphological characters for a variety. Nomenclature standards must be permanently stored in a scientific herbarium collection. The N. I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR) in alliance with national plant breeders initiated a first nomenclature standard collection of national-breeding cultivars. Aside to classical herbarising, the collection utilises molecular genetic techniques, particularly genotyping, to enable extended variety verification. The VIR team has developed the nomenclature standards of six apple varieties originated by the Crimean Experimental Breeding Station, a VIR Branch (Beloe Solntse, Zolotoy Potok, Kubanochka, Leto Krasnoe, Luchistoe and Shchedrost). The standards are supplemented with DNA barcodes obtained jointly with the N. I. Vavilov Institute of General Genetics. Fifteen microsatellite markers were used for barcoding. The herbarium nomenclature specimens adhered to the VIR-developed protocol in accordance with the International Code of Nomenclature for Cultivated Plants (ICNCP). The specimens are registered in the VIR Herbarium database and deposited in the World Crop Wild and Weed Relatives (WIR) Herbarium type collection.
Databáze: OpenAIRE