Dietary gamma-aminobutyric acid ameliorates growth impairment and intestinal dysfunction in turbot (Scophthalmus maximusL.) fed a high soybean meal diet
| Autor: | Qin-Yuan Ma, Yuan Tian, Chao-Qun Li, Bei-Li Zhang |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
medicine.medical_specialty
Antioxidant medicine.medical_treatment Soybean meal General Medicine Biology medicine.disease_cause biology.organism_classification Malondialdehyde medicine.disease gamma-Aminobutyric acid Turbot chemistry.chemical_compound Fish meal Endocrinology chemistry Internal medicine medicine Dysbiosis Oxidative stress Food Science medicine.drug |
| Zdroj: | Food & Function. 13:290-303 |
| ISSN: | 2042-650X 2042-6496 |
| Popis: | Over-substitution of fishmeal with soybean meal (SBM) commonly leads to inferior growth performance and intestinal dysfunction in fish. This study aims to evaluate whether dietary gamma-aminobutyric acid (GABA) could ameliorate the adverse effects in turbot fed a high-SBM diet (HSD). Two hundred and seventy turbots were randomly divided into three treatments fed with a control diet (CNT, containing 60% fishmeal), an HSD (with 45% fishmeal protein replaced by SBM), and an HSD supplemented with GABA (160 mg kg-1) for 53 days. The results displayed that dietary GABA ameliorated HSD-induced growth impairment and enhanced the feed intake of turbot. GABA ameliorated HSD-induced oxidative stress and apoptosis in turbot intestine by restoring the antioxidant parameters (malondialdehyde level, antioxidant enzymes activity and antioxidant mechanism-related genes expression) and expression of apoptosis-related genes (Bcl2, Bax, Bid, and Caspase3) to similar levels to those in the CNT group. GABA alleviated HSD-induced intestinal pathological disruption and inflammatory alterations with significantly decreased mRNA expression of pro-inflammatory cytokines TNF-α and IL-1β and signal molecule NF-κB p65, and elevated mRNA expression of anti-inflammatory cytokine TGF-β1. Furthermore, GABA could act as an intestinal microbiota modulator, which reversed HSD-induced microbiota dysbiosis. Spearman’s correlation analysis indicated that the altered intestinal microbiota was closely associated with the growth performance and intestinal function of turbot. Taken together, GABA ameliorated HSD-induced intestine dysfunction via relieving oxidative stress, inflammation, apoptosis and microbiota dysbiosis, and the findings would contribute to a better understanding for the function of GABA in fish intestine. |
| Databáze: | OpenAIRE |
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