| Popis: |
A method to detect the presence of diuretics, probenecid and other agents of doping interest (morazone, mesocarb, caffeine) in urine was developed. The extraction procedure was optimized to obtain adequate recoveries of the compounds under study. The best results were achieved using alkaline extraction (pH 9.5) with ethyl acetate and the salting-out effect (sodium chloride). The extracts were analysed by HPLC with diode-array detection, using a high-speed reversed-phase column and a mobile phase containing acetonitrile and 0.1 M ammonium acetate (adjusted to pH 3), with gradient elution. Under these conditions, the time of analysis per sample was 12 min. Extraction recoveries, detection limits and results of the reproducibility assays are presented. The method allows the detection of the compounds in urine during at least 24 h after a therapeutic dose of the drug. Quantitative results obtained from excretion studies are also presented. |
