The Occurrence of Glycosphingolipids Containing Mannose in the Sea-Water Bivalve, Meretrix lusoria (Hamaguri)1
| Autor: | Osamu Itasaka, Mutsumi Sugita, Takeshi Yamamura, Taro Hori, Hiromi Nakae, Yoshiko Takamiya |
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| Rok vydání: | 1985 |
| Předmět: |
chemistry.chemical_classification
Ceramide Mannose General Medicine Acidic Glycosphingolipids Biology biology.organism_classification Biochemistry carbohydrates (lipids) chemistry.chemical_compound Column chromatography Glycolipid chemistry Monosaccharide lipids (amino acids peptides and proteins) Silicic acid Meretrix lusoria Molecular Biology |
| Zdroj: | The Journal of Biochemistry. 98:27-33 |
| ISSN: | 1756-2651 0021-924X |
| DOI: | 10.1093/oxfordjournals.jbchem.a135268 |
| Popis: | Total neutral and acidic glycosphingolipids were prepared from whole tissues of the sea-water bivalve, Meretrix lusoria, and the former preparation was further fractionated into subgroups by silicic acid column chromatography. The fractions obtained as mono-(ceramide monosaccharide, CMS), di-(CDS) and triglycosylceramides (CTS) were characterized by thin-layer chromatography, partial hydrolysis with exoglycosidases, methylation studies, CrO3 oxidation, and GLC analysis of the component sugars, fatty acids and long-chain bases. The following structures are proposed: Gal-Cer and Glc-Cer for CMS, Gal(beta 1----4)Glc-Cer and Man(beta 1----4)Glc-Cer (MlOse2Cer) for CDS, Man(alpha 1----3)Man(beta 1----4)Glc-Cer (MlOse3Cer) and Gal(alpha 1----3)Man(beta 1----4)Glc-Cer (II3 alpha Gal-MlOse2Cer) for CTS. To our knowledge II3 alpha Gal-MlOse2Cer has not previously been reported. The fatty acid composition of CMS, CDS, and CTS consisted almost entirely of saturated C16-C24 acids with large amounts of 2-hydroxypalmitic acid and 2-hydroxystearic acid. The long-chain bases consisted of 4-sphingenine and 4,8-sphingadienine. More complex neutral glycolipids than CTS, as well as an acidic glycolipid, were examined by TLC and GLC of the constituent sugars, and an immunochemical technique.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
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