CD4+ T cells promoting humoral responses in mice (88.22)
| Autor: | Fouad Eddahri, Sébastien Denanglaire, Oberdan Leo, Fabienne Andris |
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| Rok vydání: | 2007 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 178:S142-S143 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.178.supp.88.22 |
| Popis: | Following antigen inoculation in naïve transgenic mice, we identified an antigen-specific CD4+ T cell subset expressing high level of both CD62L and CD44 that differ from the well characterized inflammatory cytokine producer CD62Llo CD44hi T cells. Antigen-primed CD62LhiCD44hi T cells induced extensive antibody secretion in B cell cooperation tests despite defective IL-4 and IFN-γ production, while CD62Llo CD44hi T cells secreted high amount of IL-4 and IFN-γ but did not induce optimal IgG secretion by cocultured B cells, suggesting that T cell-dependent antibody secretion by B lymphocytes (Th-B function) could be dissociated from elevated Th1 and Th2 cytokine production. We therefore studied the stimulation requirements leading to humoral and inflammatory immune responses following a dendritic cell-based immunisation protocol. The data obtained suggested that injection of antigen-pulsed mature DC primed naïve T cells for IFN-γ secretion in vivo whereas injection of antigen-pulsed immature DC did not. However, immature DC were fully capable of promoting antigen specific antibody secretion, suggesting that activation protocols described as “suboptimal” for Th cell cytokine secretion induced optimal Th-B capacities. Together, these studies should help in the development of novel vaccination strategies inducing optimal antibody responses while minimizing local inflammatory reactions. |
| Databáze: | OpenAIRE |
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