Multiple calcium channels and kinases mediate α7 nicotinic receptor neuroprotection in PC12 cells
| Autor: | Roger L. Papke, Jeffrey A. Hughes, Edwin M. Meyer, Viviana Puig, Ke Ren, Yoshitsugu Itoh |
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| Rok vydání: | 2005 |
| Předmět: |
Agonist
medicine.medical_specialty Voltage-dependent calcium channel Ryanodine receptor Chemistry medicine.drug_class p38 mitogen-activated protein kinases T-type calcium channel chemistry.chemical_element Pharmacology Calcium Biochemistry Neuroprotection Calcium in biology Cellular and Molecular Neuroscience Endocrinology Internal medicine medicine |
| Zdroj: | Journal of Neurochemistry. 94:926-933 |
| ISSN: | 1471-4159 0022-3042 |
| Popis: | α7 Nicotinic receptors are calcium permeant and provide neuroprotection against many insults. We investigated the roles of intracellular calcium ions and downstream calcium channels in this protection. The α7 agonist GTS-21 prevented pheochromocytoma cell death induced by nerve growth factor + serum deprivation over a 3-day interval. This effect was blocked by the intracellular calcium chelator 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid in a manner that did not appear to involve changes in receptor density. 1,2-Bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid blocked GTS-21-induced protein kinase C activation, a necessary process for protection. The insositol triphosphate calcium-channel blocker xestospongin C and the phospholipases C inhibitor U-73122 blocked protection, ryanodine partially attenuated protection, but the L-type channel antagonist nifedipine had no effect. ERK1/2 but not JNK and p38 were activated by GTS-21, and the ERK phosphorylation inhibitors PD98059 and U0126 blocked protection. |
| Databáze: | OpenAIRE |
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