Towards a Clinical Application of Freeze-Dried Human Platelets

Autor:	Naomi J. Walker, Yehuda Tamari, Fern Tablin, John H. Crowe, Willem F. Wolkers
Rok vydání:	2002
Předmět:	Chromatography Chemistry Vapor phase Biomedical Engineering Albumin Cell Biology Trehalose General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Platelet Platelet concentrate Mean platelet volume Blood bank Biotechnology
Zdroj:	Cell Preservation Technology. 1:175-188
ISSN:	1557-8119 1538-344X
Popis:	Storage of platelets remains a major problem for blood banks. In this study we describe the development of a lyophilization protocol for platelet concentrates obtained from a blood bank. In previous studies with small samples we reported some considerable success. We now confirm those earlier results, extend them to platelet concentrates, and scale up the process to a full unit of platelets. Platelets were loaded with trehalose and freeze-dried in a formulation of trehalose and albumin. Optimal survival rates were found, using a 1:1 weight ratio of trehalose and albumin in the lyophilization buffer. Survival rates of 90% were found when the platelets were freeze-dried in 1-mL aliquots, in vials. Freeze-drying 1 platelet concentrate unit in a specially designed lyophilization bag yielded 85% recovery after rehydration. Gradual rehydration from the vapor phase resulted in a mean platelet volume distribution similar to that of fresh control platelets, whereas directly rehydrated platelets were considerably l...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a85ce5adbdbc9c87672995b302a20d7b https://doi.org/10.1089/153834402765035617 Zobrazit plný text záznamu