An improved MS2-MCP imaging system with minimal perturbation of mRNA stability

Autor:	Weihan Li, Anna Maekiniemi, Hanae Sato, Christof Osman, Robert H. Singer
Rok vydání:	2022
Předmět:	viruses
DOI:	10.1101/2022.02.05.479257
Popis:	The MS2-MCP imaging system is widely used to study the mRNA spatial distribution in living cells. Here, we report that the MS2-MCP system may destabilize the tagged mRNA by targeting it to the nonsense-mediated mRNA decay pathway. We introduce an improved version, which has minimal perturbation of the mRNA stability.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a852adf41376e8245c31c0527c0c8c03 https://doi.org/10.1101/2022.02.05.479257 Zobrazit plný text záznamu