Dietary bisphenol A prevents ovarian degeneration and bone loss in female mice lacking the aromatase gene (Cyp19 )
| Autor: | Katsumi Toda, Teruhiko Okada, Chisato Miyaura, Yutaka Shizuta |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
endocrine system
medicine.medical_specialty biology Bone morphogenetic protein 15 urogenital system medicine.drug_class Growth factor medicine.medical_treatment Ovary Growth differentiation factor-9 medicine.disease Biochemistry medicine.anatomical_structure Endocrinology Estrogen Internal medicine Progesterone receptor medicine biology.protein Aromatase Aromatase deficiency hormones hormone substitutes and hormone antagonists |
| Zdroj: | European Journal of Biochemistry. 269:2214-2222 |
| ISSN: | 0014-2956 |
| DOI: | 10.1046/j.1432-1033.2002.02879.x |
| Popis: | We previously generated mice lacking aromatase activity by targeted disruption of Cyp19 (ArKO mice), and reported phenotypes of the female mice, showing hemorrhage formation and follicular depletion in the ovary, diminution in uterine size, and bone loss. In the present study, we examined the influence of dietary bisphenol A (BPA), a monomer used for the production of polycarbonate and known to have estrogenic activity, on these phenotypes of the ArKO mice. When ArKO mice were fed chow diets supplemented with 0.1% or 1% (w/w) BPA for 5 months, they were protected from ovarian degeneration, uterine diminution and bone loss in a dose-dependent manner. Northern blot analyses of ovarian RNA of ArKO mice showed differences in the expression levels of insulin-like growth factor (IGF)-I, IGF-I receptor, growth differentiation factor 9 and bone morphogenetic protein 15 as compared with those in the ovaries of wild-type mice. The differences in the expression levels were restored by dietary BPA. In the ArKO uteri, expression of progesterone receptor and vascular endothelial growth factor mRNAs was diminished, and was restored by BPA to the levels in wild-type mice. In contrast, BPA had little effect on the ovarian, uterine and skeletal structures of wild-type mice. In conclusion, estrogenic effects of BPA on the reproductive tract as well as skeletal tissue were evident in adult female ArKO mice. These results suggest that the ArKO mouse is an animal model suitable for studying effects of estrogenic chemicals as well as estrogen in vivo. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|