Peptide αthioester formation using standard Fmoc-chemistry

Autor:	Annette G. Beck-Sickinger, Regula von Eggelkraut-Gottanka, Annerose Klose, Michael Beyermann
Rok vydání:	2003
Předmět:	chemistry.chemical_classification chemistry Carboxylic group Organic Chemistry Drug Discovery Fmoc chemistry Peptide Epimer Thioester Biochemistry Peptide sequence Combinatorial chemistry Amino acid
Zdroj:	Tetrahedron Letters. 44:3551-3554
ISSN:	0040-4039
DOI:	10.1016/s0040-4039(03)00582-3
Popis:	A highly efficient and simple Fmoc-based preparation of peptide αthioesters is presented. After Fmoc/t-butyl solid-phase synthesis on 2-chlorotrityl resin the C-terminal carboxylic group of the protected peptide is directly converted to the corresponding thioester. The method leads to very high yields, shows a low level of epimerization and can be easily applied also for the preparation of long peptide αthioesters as demonstrated for the 41 amino acid N-terminal fragment of pro-neuropeptide Y (proNPY 1–40).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a83312e33a0cad8021a379e5bd73223e https://doi.org/10.1016/s0040-4039(03)00582-3 Zobrazit plný text záznamu Full Text from ScienceDirect