Nucleotide sequences of the microsatellite locus Du215 (arm) allelic variants in the parthenospecies Darevskia armeniaca (Lacertidae)

Autor:	D. N. Malysheva, O N Tokarskaia, Alexey P. Ryskov, A. A. Vergun, Sevast'ianova Ga, I S Darevskiĭ
Rok vydání:	2007
Předmět:	Genetics chemistry.chemical_classification biology Darevskia armeniaca Locus (genetics) biology.organism_classification Human genetics chemistry.chemical_compound chemistry Lacertidae Microsatellite Nucleotide Allele DNA
Zdroj:	Russian Journal of Genetics. 43:116-120
ISSN:	1608-3369 1022-7954
DOI:	10.1134/s1022795407020032
Popis:	Using monolocus PCR analysis with the pairs of primers designed for the Du215 locus of Darevskia unisexualis, allelic polymorphism at the orthologous locus in the populations of the related parthenospecies D. armeniaca was investigated. It was demonstrated that Du215 (arm) locus was polymorphic and in the populations of parthenospecies D. armeniaca (n = 127) represented by at least three allelic variants, differing from each other by the size and composition of microsatellite cluster, and by single nucleotide substitutions in flanking DNA. Unlike the Du215 locus, Du215 (arm) was shown contain not only GATA, but also (GACA) repeats, which were absent in D. unisexualis. Thus, in this study, the data on the molecular nature of allelic polymorphism at one of the microsatellite loci of the parthenospecies D. armeniaca were reported.
Databáze:	OpenAIRE
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