Identification of Novel Immunogenic Proteins in Mycoplasma capricolum subsp. Capripneumoniae Strain M1601

Autor:	Hai-yan Zhao, Ping Zhao, He Ying, Ke-shan Zhang, Pengcheng Gao, Chu Yuefeng, Zhong-xin Lu, Nian-Zhang Zhang, Xuan Zhang
Rok vydání:	2012
Předmět:	Gel electrophoresis Electrophoresis Matrix-assisted laser desorption/ionization General Veterinary Membrane protein biology Antigen Chemistry biology.organism_classification Polyacrylamide gel electrophoresis Homology (biology) Mycoplasma capricolum Microbiology
Zdroj:	Journal of Veterinary Medical Science. 74:1109-1115
ISSN:	1347-7439 0916-7250
Popis:	The purpose of this study was to obtain immunogenic proteins and potential proteins of interest that were isolated from Mycoplasma capricolum subsp. capripneumoniae (Mccp) by MALDI-TOF mass spectrometry. One-dimensional SDS-PAGE and two-dimensional gel electrophoresis of whole cell preparation were conducted, and membrane proteome maps were prepared by immunoblotting. One-dimensional SDS-PAGE identified three immunogenic proteins with molecular masses in the range 29-97.2 kDa, two of which were in the membrane protein fraction. After two-dimensional gel electrophoresis, 20 highly immunogenic proteins were identified in the whole cell protein preparation while 9 immunogenic proteins were identified in the membrane protein fraction. This indicated that membrane proteins were the principle immunogenic proteins in Mccp. These proteins may have potential for the development of improved diagnostic tests and possible vaccines.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a81ef098223d9ca27473cd1ee6c6db89 https://doi.org/10.1292/jvms.12-0095 Zobrazit plný text záznamu