Simple Method for Quantifying Alpha-Tocopherol in Low-Density+Very-Low-Density Lipoproteins and in High-Density Lipoproteins
| Autor: | D Bonnefont-Rousselot, S Troupel, Delattre J, S. Lepage, F David, Alain Legrand, G Carcelain, J. Peynet |
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| Rok vydání: | 1992 |
| Předmět: |
Very low-density lipoprotein
Chromatography Sodium Biochemistry (medical) Clinical Biochemistry chemistry.chemical_element High density chemistry.chemical_compound chemistry Reagent lipids (amino acids peptides and proteins) Ultracentrifuge alpha-Tocopherol Quantitative analysis (chemistry) Lipoprotein |
| Zdroj: | Clinical Chemistry. 38:1792-1795 |
| ISSN: | 1530-8561 0009-9147 |
| DOI: | 10.1093/clinchem/38.9.1792 |
| Popis: | We assessed the distribution of alpha-tocopherol in serum lipoprotein samples after separating the lipoprotein fractions by either sequential ultracentrifugation or selective precipitation with sodium phosphotungstate-magnesium chloride reagent. alpha-Tocopherol concentrations were determined by reversed-phase high-performance liquid chromatography. After ultracentrifugation, we found that in men, low- and very-low-density serum lipoproteins (LDL-VLDL) contained 53.6% of alpha-tocopherol vs 46.4% in high-density lipoproteins (HDL). In women, serum LDL-VLDL contained 45.6% alpha-tocopherol after ultracentrifugation vs 54.4% in HDL. After selective precipitation, the proportions of alpha-tocopherol in men were 56.1% in LDL-VLDL vs 43.9% in HDL, and in women, 45.4% in LDL-VLDL vs 54.6% in HDL. After selective precipitation, alpha-tocopherol recovery from whole lipoprotein fractions was 97% to 100% vs 80% after ultracentrifugation, thus allowing more accurate alpha-tocopherol quantification than after separation by ultracentrifugation. |
| Databáze: | OpenAIRE |
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