Cytologic, histologic, microbiologic, and electron microscopic characterization of a canine Prototheca wickerhamii infection
| Autor: | Robert J. Ossiboff, Mary K. Leissinger, James F. X. Wellehan, Salvatore Frasca, Bethany R. Sabatino, Albert B. Jeon, Vsevolod L. Popov, Kellie M. Whipple |
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| Rok vydání: | 2020 |
| Předmět: |
030213 general clinical medicine
medicine.medical_specialty Pathology Protothecosis General Veterinary biology 040301 veterinary sciences 04 agricultural and veterinary sciences Prototheca biology.organism_classification medicine.disease Prototheca wickerhamii Staining 0403 veterinary science Basophilic 03 medical and health sciences 0302 clinical medicine medicine Histopathology Lymph Peripheral lymph |
| Zdroj: | Veterinary Clinical Pathology. 49:326-332 |
| ISSN: | 1939-165X 0275-6382 |
| DOI: | 10.1111/vcp.12864 |
| Popis: | An adult dog was presented for chronic cough and a recent development of ulcerated, erythematous nares with nasal discharge. Cytology of enlarged peripheral lymph nodes revealed many intracellular and extracellular organisms. These round or rarely oval organisms measured approximately 5-9 µm in diameter and frequently contained several globular structures, ranging from deeply basophilic to magenta. A thin, clear halo was present. Smaller 1-2 µm, magenta forms were also observed. Fungal culture yielded small, wet, raised, irregularly shaped, white to pale tan colonies. Microbiologic staining of cultured material revealed features suggestive of algae. Histopathology of the lymph nodes revealed marked granulomatous inflammation with intralesional algal organisms suggestive of Prototheca. Electron microscopic findings were also consistent with protothecosis. Polymerase chain reaction, followed by direct DNA sequencing, identified the organism as Prototheca wickerhamii. A brief literature review discussing protothecosis in veterinary medicine is included. |
| Databáze: | OpenAIRE |
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