7-Deoxy-trans-dihydronarciclasine Isolated from Lycoris chejuensis Inhibits Neuroinflammation in Experimental Models
| Autor: | Ming-Yao Gu, Dong Zhao, Li Jun Zhang, Hyo Jin Jeon, Yong-Baik Cho, Hyun Ok Yang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
biology Microglia Chemistry medicine.medical_treatment 010401 analytical chemistry General Chemistry 01 natural sciences Molecular biology 0104 chemical sciences Nitric oxide Nitric oxide synthase Blot Interleukin 10 chemistry.chemical_compound Cytokine medicine.anatomical_structure biology.protein medicine General Agricultural and Biological Sciences Interleukin 6 Neuroinflammation 010606 plant biology & botany |
| Zdroj: | Journal of Agricultural and Food Chemistry. 67:9796-9804 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/acs.jafc.9b03307 |
| Popis: | Overactivated microglia and persistent neuroinflammation hold an important role in the pathophysiology of neurodegenerative diseases. The extract of Lycoris chejuensis (CJ) and its active compound, 7-deoxy-trans-dihydronarciclasine (named E144), attenuated expressions of pro-inflammatory factors, including nitric oxide, prostaglandin E2, inducible nitric oxide synthase, cyclooxygenase-2 (COX-2), tumor necrosis factor α (TNF-α), and interleukin 6, secreted by lipopolysaccharide-activated BV-2 microglial cells, as measured by an enzyme-linked immunosorbent assay or western blotting. In contrast, CJ extract and E144 promoted the secretion of the anti-inflammatory cytokine, interleukin 10. Moreover, we found that E144 attenuated the expression of TNF-α and COX-2 in the cerebral cortex of lipopolysaccharide-treated mice and/or T2576 transgenic mice as well as reduced the reactive immune cells visualized by ionized calcium-binding adaptor molecule 1. Our results suggest the possibility of E144 to serve as a potential anti-neuroinflammatory agent by preventing excess production of pro-inflammatory factors. |
| Databáze: | OpenAIRE |
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