A Fluorogenic Trehalose Probe for Tracking Phagocytosed Mycobacterium tuberculosis
| Autor: | Qihua Zhu, Jianghong Rao, Mireille Kamariza, Ke Jiang, Jinghang Xie, Tingting Dai, Carolyn R. Bertozzi |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
congenital
hereditary and neonatal diseases and abnormalities Tuberculosis biology Phagocytosis General Chemistry Pathogenic mycobacterium 010402 general chemistry biology.organism_classification medicine.disease 01 natural sciences Biochemistry Trehalose Catalysis Bacterial cell structure 0104 chemical sciences Microbiology Mycobacterium tuberculosis chemistry.chemical_compound Metabolic pathway Colloid and Surface Chemistry chemistry Corynebacterineae medicine |
| Zdroj: | Journal of the American Chemical Society. 142:15259-15264 |
| ISSN: | 1520-5126 0002-7863 |
| DOI: | 10.1021/jacs.0c07700 |
| Popis: | Tuberculosis (TB) disease is a global epidemic caused by the pathogenic Mycobacterium tuberculosis (Mtb). Tools that can track the replication status of viable Mtb cells within macrophages are vital for the elucidation of host-pathogen interactions. Here, we present a cephalosphorinase-dependent green trehalose (CDG-Tre) fluorogenic probe that enables fluorescence labeling of single live Bacille Calmette-Guerin (BCG) cells within macrophages at concentrations as low as 2 μM. CDG-Tre fluoresces upon activation by BlaC, the β-lactamase uniquely expressed by Mtb, and the fluorescent product is subsequently incorporated within the bacterial cell wall via trehalose metabolic pathway. CDG-Tre showed high selectivity for mycobacteria over other clinically prevalent species in the Corynebacterineae suborder. The unique labeling strategy of BCG by CDG-Tre provides a versatile tool for tracking Mtb in both pre- and postphagocytosis and elucidating fundamental physiological and pathological processes related to the mycomembrane. |
| Databáze: | OpenAIRE |
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