| Popis: |
The effects of adding Megasphaera elsdenii (ME) to equine cecal microorganisms provided oligofructose (inulin [IN]) or corn starch (CS) were evaluated. Four cecally cannulated horses (529 ± 70 kg BW) were used as donors of cecal inoculum for randomized complete block experiments with 2 × 2 factorial treatment arrangements. Factor 1 was carbohydrate source (IN or CS). Factor 2 was level of added ME [7.93 × 108 CFU (study 1; with ME), 2 × 107 CFU (study 2; with ME), or 0 CFU]. In study 1, 150 mL cultures were incubated for 48 hours. In study 2, 20 mL cultures were incubated for 0, 3, 6, 9, 12, 15, 18, 21, 24, 30, 36, 42, and 48 hours. In study 1, gas production ceased after 8 hours in cultures provided IN without ME, whereas other cultures remained active for 48 hours. Cultures containing IN yielded greater lactate (P .05), ME reduced lactate accumulation, stabilized pH, and enhanced fermentative activity in study 1. |
Full Text from ScienceDirect