Enhancement Antimicrobial Activity of Clarithromycin by Amine Functionalized Mesoporous Silica Nanoparticles as Drug Delivery System
| Autor: | Hamid Akbari Javar, Mehdi Shafiee Ardestani, Mehdi Khoobi, Mohammad Porgham Daryasari, Massoud Amanlou, Malihe Hassanzadeh, Payam Ghasemi-Dehkordi, Pegah Khosravian |
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| Rok vydání: | 2018 |
| Předmět: |
Biodistribution
Chemistry Pharmaceutical Science 02 engineering and technology Mesoporous silica 010402 general chemistry 021001 nanoscience & nanotechnology Antimicrobial medicine.disease_cause 01 natural sciences Fluorescence 0104 chemical sciences In vivo Staphylococcus aureus Drug Discovery Drug delivery medicine Molecular Medicine 0210 nano-technology Ex vivo Nuclear chemistry |
| Zdroj: | Letters in Drug Design & Discovery. 15:787-795 |
| ISSN: | 1570-1808 |
| DOI: | 10.2174/1570180815666180117155818 |
| Popis: | Methods: A large pore amine functionalized MSN (MSNs-NH2) is described here to facilitate delivery of clarithromycin (CLM) as an antibacterial drug and enhance the efficacy against Gram positive and Gram negative bacterial samples. Prepared particles were characterized by Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), nitrogen adsorption/desorption isotherms, Fourier Transform Infrared (FT-IR) spectroscopy and X-ray Diffraction (XRD) spectroscopy. The antimicrobial activity was evaluated by agar well diffusion and broth dilution methods. Therefore, the biodistribution of FITC-MSNs was investigated by measure the NIR intensity fluorescent of fluorescent images from whole animal and dissected organs of NMRI mice. Results: The results showed that the CLM loaded MSNs-NH2 (CLM/MSNs-NH2) were successfully prepared having good payload and pH-sensitive drug release kinetics. The antimicrobial investigation against Staphylococcus aureus and Escherichia coli was showed better performance of antimicrobial activity of these nanoparticles. In vivo and ex vivo fluorescent imaging investigation on NMRI mice were shown that FITC-MSNs-NH2 accumulated in the liver and kidney and notably in lung tissue. Conclusion: The CLM/MSNs-NH2 exhibited higher antimicrobial activity and enhanced the possibility of microbial infection therapy especially at respiratory infections |
| Databáze: | OpenAIRE |
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