Phospholipid profiling of 57 soybean (Glycine max) varieties by high-performance liquid chromatography-tandem mass spectrometry and principal component analysis to classify Korean soybean germplasm
| Autor: | Gon-Sup Kim, Soo Taek Kim, Yun-Hi Kim, Jong Il Chung, Chi Yeon Kim, Sung Chul Shin, Soo-Jung Lee, Sung Woo Jeong, Jae-Han Shim, Semin Park |
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| Rok vydání: | 2012 |
| Předmět: |
Pharmacology
Germplasm Chromatography Chemistry Clinical Biochemistry Phospholipid General Medicine Repeatability Mass spectrometry Tandem mass spectrometry Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound Drug Discovery Principal component analysis Molecular Biology |
| Zdroj: | Biomedical Chromatography. 27:27-33 |
| ISSN: | 0269-3879 |
| DOI: | 10.1002/bmc.2743 |
| Popis: | Phospholipids (PLs) in 57 varieties of soybeans were profiled by high-performance liquid chromatography–electrospray ionization-tandem mass spectrometry and principal component analysis (PCA) to discriminate PL-rich soybeans. The PL calibration curves showed linearity with correlation coefficients >0.9964. The recoveries at 5 mg/L spiked level ranged from 72.8 to 86.7% and those at 12.5 mg/L from 78.2 to 85.1%. The repeatability at a 5 mg/L spiked level ranged from 2.5 to 7.0% and those at 12.5 mg/L from 1.2 to 3.9%. The average total PL content in the 57 soybean varieties was about 35.3 mg/kg. The total PL content was the highest in Aodaiz (35, 48.7 ± 1.4 mg/kg) and the lowest in Poongsannamul (56, 16.0 ± 0.7 mg/kg). The PCA showed that RS-78sun (42), Gyeongsang #1 (3) and Aodaiz (35) are the most improved varieties of the investigated 57 varieties from the viewpoint of PL content. Copyright © 2012 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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