Investigation of the kinetics of selenium accumulation byLentinula edodes(Berk.) mycelial culture by use of reversed-phase high-performance liquid chromatography with fluorimetric detection

Autor:	I. Łuczak, J. Turło, Franciszek Herold, Bożenna Gutkowska
Rok vydání:	2009
Předmět:	Chromatography biology fungi chemistry.chemical_element Industrial fermentation General Chemistry equipment and supplies biology.organism_classification High-performance liquid chromatography Yeast chemistry.chemical_compound Lentinula chemistry Medicinal fungi Derivatization Mycelium Selenium
Zdroj:	Acta Chromatographica. 21:1-11
ISSN:	2083-5736 1233-2356
DOI:	10.1556/achrom.21.2009.1.1
Popis:	Summary We hypothesized that extracts from selenium (Se)-enriched Lentinula edodes mycelia would have higher anticancer and immunostimulating properties than the nonenriched extracts currently used to treat cancer. Previously, we demonstrated that submerged cultivated mycelia of L. edodes accumulated Se from the cultivation medium very efficiently, more so than yeast. The objective of this study was to investigate the kinetics of Se accumulation by L. edodes mycelial culture. The L. edodes mycelia were cultivated in medium enriched with 20 μg mL−1 Se in a 10-L jar fermenter. Each day, a 100-mL sample was taken from the fermenter and the concentration of Se in the dried mycelia, in the filtered medium, and in the wash water was determined by reversed-phase high-performance liquid chromatography (RP HPLC). We adapted a widely used fluorimetric method based on derivatization with 2,3-diaminonaphthalene for analysis of Se for RP HPLC. Se recovery was determined on the basis of the theoretical amount of Se in ...
Databáze:	OpenAIRE
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