Isolation and characterization of enteropathogenic and enterotoxinogenic Escherichia coli from dairy products consumed in Burkina Faso
| Autor: | Bissoume Sambe-Ba, Abdoul Aziz Wane, Touwendsida Serge Bagre, Evariste Bako, René Dembélé, Gertrude Bsadjo Tchamba, Alfred S. Traore, Hadiza Bawa Ibrahim, Amy Gassama-Sow, Nicolas Barro |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Nalidixic acid Tetracycline medicine.drug_class 030106 microbiology Antibiotics Pasteurization Plant Science Amoxicillin Biology medicine.disease_cause Microbiology law.invention 03 medical and health sciences 030104 developmental biology Infectious Diseases Antibiotic resistance law Ticarcillin medicine Escherichia coli medicine.drug |
| Zdroj: | African Journal of Microbiology Research. 11:537-545 |
| ISSN: | 1996-0808 |
| Popis: | Food-borne diseases represent a public major health problem, and drink-water, juice, meat, and milk products are usually involved. This study aimed to evaluate the antibiotic resistance of diarrheagenic E. coli isolated from dairy products consumed in Burkina Faso. Five hundred and twenty-two samples were gathered. Escherichia coli were isolated using Standard Microbiological Methods. A 16-plex polymerase chain reaction for virulence associated genes was applied. The standard disc diffusion methods were used to assess the susceptibility to 31 antibiotics. Classes 1, 2, 3 integrons were categorized using PCR. Results showed 1.92% (10/522) of milk products was contaminated by diarrheagenic E. coli. Enterotoxinogenic E. coli was found in 4.45% (4/89) of curds, 3.4% (3/88) of pasteurized milk, and 1.15% (1/87) of “degue”. Also, “degue” was contaminated at 2.3% (2/87) by atypical enteropathogenic E. coli. Antibiogram susceptibility showed that pathogenic isolated resists mainly to tetracycline, amoxicillin, ticarcillin, nalidixic acid, sulfonamide, and trimethoprim-sulfamethoxazole. Only the class 1 integrons was detected in 80% of diarrheagenic E. coli. Among this class 1 integrons, 4 strains contains a variable region, and the subsequent result showed a presence of dfrA7 gene coding for trimethoprim resistance. It appears in this study that dairy products are contaminated by enteropathogenic and enterotoxinogenic E. coli, which are resistant to antibiotics frequently used. This study therefore recommends the training of milk products transformers. Key words: Dairy products, diarrheagenic Escherichia coli, antibiotics resistance, integrons, Burkina Faso. |
| Databáze: | OpenAIRE |
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