Mycotoxin-induced cytotoxicity of commercially available pelleted feline feed in feline peripheral blood mononuclear cells Ex Vivo

Autor:	C. Tiloke, S.D. Singh, S. Baijnath, A. Phulukdaree, N.S. Abdul, A.A. Chuturgoon
Rok vydání:	2020
Předmět:	Antioxidant Chemistry Thiobarbituric acid medicine.medical_treatment Glutathione Peripheral blood mononuclear cell Molecular biology Lipid peroxidation chemistry.chemical_compound Food Animals Apoptosis medicine TBARS Mycotoxin
Zdroj:	Animal Nutrition and Feed Technology. 20:217
ISSN:	0974-181X 0972-2963
Popis:	Mycotoxin contamination is a major concern in developing countries such as South Africa, with pelleted pet foods often being contaminated with mycotoxins that have a negative impact on pet health. This study investigated the cytotoxicity of some common mycotoxins found in commercial cat food. A total of 12 cat food samples consisting of six samples each from standard grocery store lines (SB) and premium veterinarian lines (PB) were collected from supermarkets and veterinary practices, respectively. Samples of pelleted cat food were extracted for mycotoxins. Cat peripheral blood mononuclear cells (PBMCs) were isolated and treated with various feed extracts for 24 h to determine the oxidative stress and antioxidant status, using the thiobarbituric acid (TBARS) and glutathione assays, mitochondrial integrity and cell death using caspsase 3/7 luminometry and Annexin V flow cytometry assays, respectively. Both PB and SB extracts decreased ATP levels (P
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a7515b34b0b29053c0b8f28834e32468 https://doi.org/10.5958/0974-181x.2020.00020.7 Zobrazit plný text záznamu