e0186 Study on the mechanism of inhibitory effect of CTLA-4Ig fusion protein on atherosclerosis in apoe deficient mice
| Autor: | Chen Jie, Zheng Dongdan, Xiong Yan, Li Xin, Liao Xiao-xing, Li Yu-Jie |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Apolipoprotein E
Tunica media medicine.medical_specialty Aorta Pentobarbital Apolipoprotein B biology business.industry medicine.medical_treatment Intraperitoneal injection Lumen (anatomy) Fusion protein Surgery Endocrinology medicine.anatomical_structure medicine.artery Internal medicine medicine biology.protein Cardiology and Cardiovascular Medicine business medicine.drug |
| Zdroj: | Heart. 96:A59-A60 |
| ISSN: | 1355-6037 |
| Popis: | Objective To investigate the mechanism of inhibitory effect of CTLA-4Ig fusion protein on atherosclerosis in mice with an apolipoprotein-E gene defect fed on cholesterol diet. Methods 30 male 10-week-old apoE(-/-) mice were fed on cholesterol diet and divided into CTLA-4Ig treatment group, IgG1 group and PBS group at random, 10 in each. The three groups were given intraperitoneal injection of CTLA-4Ig (10 μg per time), Rat-IgG1 (10 μg per time), (and) PBS (100 μl per time) respectively, twice a week, for 12 weeks. Followed by a 12-week treatment, the whole aorta from the root to crotch of iliac artery was separated after anaesthesia with the intraperitoneal injection of 1% pentobarbital and the whole (total) blood was taken to obtain serum. Subsequently, the area ratio of plaque and lumen, the thickness ratio of endangium and tunica media, the lipid-soaking extent intra-plaque and the content of collagen fibrils and smooth muscle cells intra-plaque were analysed by image-processing soft. The serum concentration of total cholesterol, CRP, sICAM-1, IFN-γ, IL-10, and TGF-β1 were measured. Results There were typical atherosclerotic plaque in apoE(-/-) mice fed on cholesterol diet after 12 weeks and it was light in the CTLA-4Ig group. There were statistical value of difference in the area ratio of plaque and lumen, the thickness ratio of endangium and tunica media, the lipid-soaking extent intra-plaque, and the content of collagen fibrils in three groups (p all 0.05). There were no significant difference in content of smooth muscle cells in three groups (p>0.05). There were no significant difference in serum concentration of total cholesterol in three groups (p>0.05). There were statistical value of difference in the serum concentration of CRP, sICAM-1, IFN-γ, IL-10, and TGF-β1 in all three groups (p all Conclusions CTLA-4Ig fusion protein could inhibit the (del) atherosclerosis progression in apoE(-/-) mice fed on cholesterol diet and it9s effect might be associated with blocking B7/CD28, anti-inflammation, promoting Th2 polarisation and affecting regulate T cells. |
| Databáze: | OpenAIRE |
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