SENSITIVE DETERMINATION OF URINARY MERCURY(II) BY A BIOLUMINESCENT TRANSGENIC BACTERIA-BASED BIOSENSOR
| Autor: | Mara Mirasoli, Patrizia Pasini, Mario Baraldini, Monica Musiani, Massimo Guardigli, Aldo Roda, Carmela Russo |
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| Rok vydání: | 2001 |
| Předmět: |
Detection limit
Chromatography Biochemistry (medical) Clinical Biochemistry Analytical chemistry chemistry.chemical_element medicine.disease_cause Biochemistry Luciferin Analytical Chemistry Mercury (element) chemistry Electrochemistry medicine Bioluminescence Luciferase Light emission Escherichia coli Biosensor Spectroscopy |
| Zdroj: | Analytical Letters. 34:29-41 |
| ISSN: | 1532-236X 0003-2719 |
| DOI: | 10.1081/al-100002702 |
| Popis: | We used a luminescence-based biosensor to determine mercury in urine samples of subjects with or without dental amalgam restorations. The system utilizes Escherichia coli as a host organism and firefly luciferase luc gene as a reporter under the control of the mercury-inducible promoter from the mer operon from transposon Tn21. The presence of mercury induces the specific gene sequence with consequent synthesis of the luciferase enzyme, and luciferin/luciferase-mediated light output occurs. The method showed a linear relationship between the light signal intensity and the Hg2+ concentration in the range of 1.67 × 10−13–1.67 × 10−7 M with a detection limit of 1.67 × 10−13 M, corresponding to 4.0 × 10−18 mol/tube. The system proved precise, accurate, and highly selective for mercury and methylmercury, with no light emission induced by other heavy metals; the matrix effect caused by urine components was minimal. The analysis of urine specimens showed that urinary mercury levels in subjects with amalgam filli... |
| Databáze: | OpenAIRE |
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