Somatic embryogenesis of Pinus sylvestris

Autor:	S. von Arnold, K. Keinonen-Mettaelae, K. von Weissenberg, Paula Jalonen, P. Eurola
Rok vydání:	1996
Předmět:	0106 biological sciences 0303 health sciences Somatic embryogenesis Polyembryony Forestry Embryo Embryo culture Biology 01 natural sciences 03 medical and health sciences Human fertilization Micropropagation Botany Ovule 030304 developmental biology 010606 plant biology & botany Explant culture
Zdroj:	Scandinavian Journal of Forest Research. 11:242-250
ISSN:	1651-1891 0282-7581
DOI:	10.1080/02827589609382933
Popis:	Embryogenic cultures (EC) of Scots pine (Pinus sylvestris L.) were initiated from immature embryos. Whole ovules were used as expiants. The responsive period for initiation began just after fertilization and remained throughout the development of first stages of early embryos. The main part of the embryogenic cultures were initiated by the time of cleavage polyembryony. Strong correlation was obtained between degree days and the responsive period. During subsequent years the experiments were repeated in Finland and Sweden. In all cases the responsive period for initiating embryogenic cultures was the same, about two weeks after fertilization. In 1991–1993, a total of 138 clones of elite pine trees were tested for their ability to initiate embryogenic cultures. Of these, 33% were responsive under our experimental conditions. Based on about 300 ovules per clone the number of embryogenic lines induced by the responding clones varied from 0.2% to 9.0% of the expiants. Several nutrient media were found to be s...
Databáze:	OpenAIRE
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