Confocal fluorescence microscopy for minimal-invasive tumor diagnosis
| Autor: | M. H. Götz, M. Zenzinger, Josef F. Bille, Stefan Fischer |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Materials science
Microscope Physics and Astronomy (miscellaneous) medicine.diagnostic_test business.industry Confocal General Engineering General Physics and Astronomy Immunofluorescence Fluorescence Signal law.invention Ultrashort laser Optics law Light sheet fluorescence microscopy Fluorescence microscope medicine business Biomedical engineering |
| Zdroj: | Applied Physics B: Lasers and Optics. 70:281-286 |
| ISSN: | 1432-0649 0946-2171 |
| DOI: | 10.1007/s003400050046 |
| Popis: | The goal of the project “stereotactic laser-neurosurgery” is the development of a system for careful and minimal-invasive resection of brain tumors with ultrashort laser pulses through a thin probe. A confocal laser-scanning-microscope is integrated in the probe. In this paper, the simulation of its optical properties by a laboratory setup and the expansion by the ability for fluorescence microscopy are reported. For a valuation of the imaging properties, the point-spread-function in three dimensions and the axial depth-transfer-function were measured and thus, among other things, the resolving power and the capacity for depth discrimination were analysed. The microscope will enable intra-operative detection of tumor cells by the method of immunofluorescence. As a first model of the application in the brain, cell cultures, that fluorescein-labelled antibodies were bound to specifically, were used in this work. Due to the fluorescence signal, it was possible to detect and identify clearly the areas that had been marked in this manner, proving the suitability of the setup for minimal-invasive tumor diagnosis. |
| Databáze: | OpenAIRE |
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