Granulocyte colony-stimulating factor receptors in human acute myelocytic leukemia
| Autor: | LM Budel, IP Touw, R Delwel, B Lowenberg |
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| Rok vydání: | 1989 |
| Předmět: | |
| Zdroj: | Blood. 74:2668-2673 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood.v74.8.2668.bloodjournal7482668 |
| Popis: | The binding of granulocyte colony-stimulating factor (G-CSF) to normal and human acute myeloid leukemia (AML) cells was investigated with radiolabeled recombinant human G-CSF (rhG-CSF). In all 14 cases of primary AML specific receptors for G-CSF were demonstrated on purified blast cells. The average numbers of G-CSF receptors ranged from very low to 428 receptors per cell (mean). Normal granulocytes showed G-CSF binding sites on their surface at higher densities (703 to 1,296 sites per cell). G-CSF receptors appeared to be of a single affinity type with a dissociation constant (kd) ranging between 214 and 378 pmol/L for AML blasts and 405 to 648 pmol/L for granulocytes. In 12 of 14 cases, including those with relatively low specific binding, G-CSF was a potent inducer of DNA synthesis of blasts in vitro; therefore, apparently relatively few receptors are required to permit activation of AML cell growth. However, in two cases cell cycling was not activated in response to G-CSF despite G-CSF receptor availability. The results show that G-CSF receptors of high affinity are frequently expressed on the blasts of human AML, but their presence may not be a strict indicator of the proliferative responsiveness of the cells to G- CSF. |
| Databáze: | OpenAIRE |
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