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The article presents the results of research work on optimizing the composition of the nutrientmedium of raspberry varieties “Bryanskaya Divo” and “Polka” for clonal micropropagation. Studies haveshown that the sterilizing agent and the time of explant processing are also important for isolating explantsof raspberry varieties. As a result of the study, the effectiveness of treatment with 0.1% mercury(II) chlorideagainst saprophytic microflora for 5 minutes is known. Murashige and Skoog (MS) medium, containing 0.5mg/l of benzylaminopurine (BAA), 0.1 mg/l of indolyl-3-fatty acid (IMS), 0.2 mg/l of gibberellic acid (HA),0.1 mg/l of thiamine (vitamin B1), 0.5 mg/l of pyridoxine (vitamin B6) and nicotinic acid (vitamin PP), 1 mg/lof ascorbic acid, 30 g/l sucrose, 100 mg/l mesoinosite, 2 mg/l glycine, and 10 mg/l iron chelate. are usedas the nutrient medium necessary for the introduction of plants in vitro. The average rate of regeneration ofexplants sterilized and introduced into the culture medium was 95%. During the research work, taking intoaccount the possibility of the presence of latent pathogenic microflora in explants, we examined them in aspecial nutrient medium Viss, poured into Petri dishes. Bacterial damage in the specified medium containing10 g / l sucrose, 15 g/l MdSO47H2O, 8 g/l casein hydrolysate, 2 g/l KN2RO4, 4 g/l yeast extract and 6 g / lgelwright was 4-5%. The MS medium containing 0.5 mg/l, 0.1 mg/l IMS, 100 mg/l mesoinosite, 2 mg/lglycine, and 5 mg/l iron chelate was optimal and effective for clonal micropropagation of raspberry varieties. |
