Activity of phase I and phase II detoxication enzymes in Antarctic and Arctic macroalgae
| Autor: | H. Sandermann, Christian Wiencke, Stephan Pflugmacher |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
chemistry.chemical_classification
0303 health sciences biology Fatty acid General Medicine Chlorophyta 010501 environmental sciences Aquatic Science Monooxygenase Oceanography biology.organism_classification 01 natural sciences Pollution Lauric acid Enzyme assay Palmitic acid 03 medical and health sciences chemistry.chemical_compound chemistry Biochemistry biology.protein Stearic acid Xenobiotic 030304 developmental biology 0105 earth and related environmental sciences |
| Zdroj: | Marine Environmental Research. 48:23-36 |
| ISSN: | 0141-1136 |
| Popis: | Presented data demonstrate the existence of various phase I and phase II detoxication enzymes in nine species of Antarctic and Arctic macroalgae cultivated under laboratory conditions. The different enzyme activities were widely distributed in the tested Chlorophyta, Phaeophyta and Rhodophyta. Concerning the cytochrome P-450 monooxygenase system, it was possible to determine several activities for fatty acid substrates such as lauric acid, palmitic acid and stearic acid and furthermore for 3-chlorobiphenyl, a xenobiotic substrate. The conversion of the 3-chlorobiphenyl to a monohydroxymonochlorobiphenyl was established using gas chromatography–mass spectrometry (GC–MS). Also phase II enzyme activity such as microsomal and soluble glutathione S -transferases and O - and N -glucosyltransferases were detected in these marine algae. These results point to similarities with detoxication in mammalian liver and confirm the concept of the `green liver' described previously and extend this concept also to marine plants, which may be important as a sink because of their large biomass. © |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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